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Both methods utilize the property of S-system models within Biochemical Systems Theory (BST) that steady states can be explicitly represented as systems of linear algebraic equations.

Both methods utilize the transverse update technique developed in the 2D USM algorithm to account for transverse fluxes without solving intermediate Riemann problems, which in turn gives cost-effective 3D methods by reducing the total number of Riemann solves.

Both methods utilize the High-Resolution Melting (HRM) technology [ 17].

While both methods utilize the rather straightforward notion of adjusting reactions' bounds according to expression levels, few key differences between them help PRIME generate more accurate models: (1) since modifying the reactions' bounds is considered to be a hard constraint, one should aim to avoid over-constraining the network based on irrelevant or noisy information.

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Both methods utilized the Novo Link polymer (Leica Microsystems, Wetzlar, Germany) and DAB + Chromogen (Dako, CA, USA) detection systems prior to hematoxylin counterstaining.

Both methods utilize low coverage WGS of the plasma cfDNA, while leveraging the large number of samples.

The methods utilize the Hilbert transform in envelope extraction.

Computerised methods utilize the whole NMR spectrum and include unnoticed (or unknown) compounds.

To facilitate direct comparison, all methods utilize the same fossil calibrations and tree topologies outlined above.

Both methods utilize exhaustive chemical reactions between the analyte and added reagents.

Both methods utilize enzyme gene expression data to constrain the maximum flux through the corresponding metabolic reactions at steady state.

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