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Furthermore, our results demonstrate a decrease in airway neutrophil recruitment in response to fixed conidia, and an increase in eosinophils in response to heat-inactivated conidia, suggesting that both methods of inactivation also differ in their stimulation of inflammatory responses.
IFN-γ-producing CD4+ T cells were significantly reduced only in response to heat-inactivated conidia (Figure 4B, right panels), while both methods of inactivation resulted in decreased IFN-γ-producing BALF CD8+ T cells (Figure 4C, right panels).
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Although the discovery of light-activated antimicrobial agents had been reported in the 1900s, only more recently research work has been developed toward the use of photodynamic process as an alternative to more conventional methods of inactivation of micro organisms).
He planned to use the tried-and-true method of inactivation to make a vaccine.
As an alternative method of inactivation, conidia were autoclaved for 30 minutes [19].
Gamma-irradiation is the preferred method of inactivation of highly infectious agents for biochemical analysis, including Ebola, Marburg and Lassa viruses [6], [7], [8].
It also became apparent that the method of inactivation was highly important for the degree of interference, with UV inactivation being far superior to heat or formalin treatment.
Aberrant promoter hypermethylation may therefore be the method of inactivation in the subset of 10 12% ccRCC negative by IHC for PBRM1 or BAP1 that show no evidence of point mutation and, also, for the inactivation of the second allele in some tumors with point mutation.
Gene inactivation: Two different methods of gene inactivation were applied.
Multiple methods of phage inactivation were tested, including drying, freezing, autoclaving, heating, and UV irradiation.
Due to photocatalytic properties, TiO2 and ZnO are applied, after powdering to NPs, in AOPs- and UV-radiation-based methods of pathogens inactivation and organic pollutants decomposition [43].
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