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To correct for differential relaxation, an inversion recovery experiment using an array of delay times from 0 to 10 s was performed on both medium and cell extracts.
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To estimate viral yields, infections were done at a moi of 1 2 pfu/cell and two days later both medium and cells were recovered, frozen and thawed three times, centrifuged for 10 min at 8,000×g and virus in the supernatant was titrated in BHK21 cells.
Briefly, both medium and cells were collected from each well and 50 μl of cell suspension were transferred to a 96-well plate in duplicate.
Relative changes in the concentrations of about 90 metabolites were monitored in both the medium and cell lysates within a whole replication cycle, with measurements every 8 h [ 30].
In contrast, MMP-2 levels were significantly decreased in cisplatin-treated cells both in conditioned medium and cell extracts.
size marker; however, the proteins detected in both the cell medium and cell extract migrated slower (lane 3).
TRAP has recently been analyzed in cell cultures and was detected in both the supernatant medium and cell lysates [ 14].
To mechanistically interrogate RIBE responses and to gain novel insights into RIBE specifically in hSC compartment, both medium transfer and cell co-culture bystander protocols were employed.
When only mature ROL (mROL) without the prosequence fused to the pre-α-factor leader sequence was expressed in S. cerevisiae, the enzyme activity was not observed in both the medium and cells.
29 markers were discovered in both conditioned medium and cell-membrane preparations derived from lung cancer cell lines.
The neuronal differentiation with detectable Map2 positivity was observed as early as 5 6 dpp in both medium and high density plated cells (Fig. 6A, B) that became quite extensive by 9 dpp (Fig. 6C F).
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