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For the flexible biorefinery, it must be suitable for both maximum ethanol and maximum electric energy production, which makes its investment costs the highest.
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Dynamic metabolic flux analysis clearly shows that the fluxes from pyruvate to lactate and formate are both strictly constrained throughout the process and that both the maximum ethanol yield (0.46C/C) and the maximum specific productivity (19.8 mmol C (g DCW −1 h−1) occur at late-exponential growth phase.
After simultaneous saccharification and fermentation (SSF), the maximum ethanol yield and concentration were 0.88 and 29.4 g/l respectively.
Validated results indicated a maximum ethanol concentration and yield of 21.96 g/l and 84.65% respectively, which were closer to the predicted optimum responses.
The maximum ethanol concentration and yield were 17.9 g/L and 53% yield, respectively, after 120 hours.
The maximum ethanol titer and productivity reached by the optimized co-culture was 46 and 0.49 g/l h, respectively.
The maximum ethanol concentration and ethanol yield from the initial glucan reached 7.5 g/l and 33% of the theoretical yield in 72 hours, respectively.
At that time the maximum ethanol concentration and ethanol yield coefficient of GX-UN120 were 67.6 g/L and 0.45 g/g, respectively, and those of Angel were 67.7 g/L and 0.45 g/g, respectively.
The maximum ethanol concentration and volumetric ethanol productivity obtained using ALM-immobilized cells under the optimal conditions were 97.54 g/L and 1.36 g/L h, respectively.
The fed-batch SSF in the presence of 3.0 g/L A-LDs at 38 °C gave the maximum ethanol production and a high enzyme recovery rate.
Medium engineering with 0.1% yeast extract and peptone, stimulated co-fermentation potential of both strains yielding maximum ethanol 0.25 g g−1 on mixed sugars with ~ 50% fermentation efficiency.
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CEO of Professional Science Editing for Scientists @ prosciediting.com