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Among the Binding SPLN deletion strains, 8% (7/88) are significantly long-lived in both mating types and 15.9% (14/88) are significantly long-lived when data from both mating types are pooled, compared to only 2.7% (15/564) and 4.3% (24) in each category for the randomly selected set of 564 deletion strains (Table 1).
The same manipulations were performed in both mating types and all microscopy experiments were conducted in diploid strains (Mut A: EY2969/ASH191; Mut B: EY2967/ASH189).
This is consistent with the fact that the pheromone response pathway is common to both mating types, and most of the proteins required in the differentiation pathway perform similar functions in the two cell types.
In all numerical calculations the initial populations were 10 pre-zygote vegetative cells of both mating types and the pan-sexual type, all other states had 0 initial abundances.
At time 0 we have populated 2% of the sites by pre-zygote vegetative individuals of both mating types and the pan-sexual type, assigning individuals to sites at random.
Homozygous diploid deletion strains (BY4741/BY4742) were sporulated, tetrad were dissected, and one or two spores from each of the four possible progeny, lys2 -/ met15 -, LYS2 / MET15, LYS2 / met15 -, and lys2 -/ MET15, were obtained for both mating types and mated to M22 (SAM2, UBI4 ), YPS163 (RAV2, MRPL22 ), or YJM326 (COQ10 ).
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Although cluster 1 included only isolates of the A1 mating type, whereas cluster 2 included both mating types (A1 and A2), HE and Ar were higher for cluster 1 than for cluster 2, especially in analyses performed without clonal copies (Table 3).
The diversity of this cluster is further illustrated by the absence of clonal lineages and the presence of both mating types (A1 and A2) in a more or less equal ratio (A1/A2 = 161/197) and multiple mt haplotypes (Ia, IIa, and Ib).
The latter occurs when both mating types, MATa and MATα, secrete a pheromone (a-factor or α-factor, respectively) to which the opposite mating type expresses the cognate G protein-coupled receptor (GPCR).
Of the 24 AD hybrid isolates with designated serotype/mating type, seven had only one mating type (MATα; αADα) and 17 had both mating types (12 aADα and 5 αAD a) present in their diploid genomes.
Specifically, sexual spores and vegetative cells in this taxon contain haploid nuclei of both mating types (mat A and mat a), resulting in heterokaryotic individuals capable of completing the life-cycle on their own; a mating-system called pseudohomothallism [ 3, 4].
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both character types and
both prey types and
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both edge types and
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both homeowner types and
both mouse types and
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