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Observed and expected heterozygosity for both markers were calculated by Arlequin.
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Cells expressing each one or both markers were manually quantified and normalized to the total amount of Hoechst+ nuclei in the field and expressed as the means±S.E.M. Number of cells lacking the expression of both markers was calculated.
The sex-specific LG maps are presented in Figure 1 and Figure 2. Intervals between adjacent markers were calculated in centimorgan units for both sex-specific maps using Kosambi mapping function.
Marker mean differences and the amount of variance explained by significant markers were calculated using true markers and not pseudomarkers.
Patient-specific risk estimates for CHD based on these two "markers" were calculated.
The average contents for all markers were calculated for each determination, and the values are indicated in Table 2.
Following manual digitisation, marker coordinates were reconstructed and their resultant displacements and maximum change in separation distance between skin and garment markers were calculated.
The recovery of the known added amount of markers added to the samples with known concentrations of markers were calculated and expressed as percentages of recovery.
The sensitivity, specificity, and Youden's index of single markers and combination markers were calculated.
Estimates of allelic effects of the markers were calculated as the mean of the posterior distributions.
Furthermore, best cut-off values for the single markers were calculated using Youden's statistics [ 20].
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