Haplotype networks were reconstructed for both genetic markers using the median-joining algorithm implemented in NETWORK v 4.510 [ 98].
We used the web-server of ABGD http://wwwabi.snv.jussieu.fr/public/abgd/abgdweb.html and analyzed both mitochondrial markers using the two available models: Jukes-Cantor (JC69) and the Kimura K80 model.
40648_2018_105_MOESM1_ESM.mp4 Additional file 1. Whole-body motion to reach with both hands two AR markers using the proposed method.
The relative expression of each target gene was normalized to GADPH for nonfusion markers, or both GAPDH and PSA for fusion markers using the comparative Ct method (Applied Biosystems User Bulletin 2, Foster City, CA).
We also determined associations between tumor groups with both clinical features and IHC markers using the chi square test, with the two-tailed Fisher's exact test correction when needed.
We developed the SPL14-04SNP marker using the ST12-specific SNP to select the OsSPL14-ST12 allele.
The sequences were retrieved for all the markers using both the forward and reverse primers.
The estimated fraction of the genetic variance explained by the markers using separately both relationships matrices followed the same trends but the results were underestimated.
exhibits reciprocal monophyly for both mitochondrial markers (and using the tropomyosine intron marker on a subsample of individuals) with all other groups/taxa.
