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The combination of both markers is a challenging task for two reasons.
These test results indicate that the combination of both markers is a reliable system to determine flower phenotype.
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As shown, an even distribution of both markers is observed in small spheroids, while a clear gradient of both Fucci-red and Fucci-green is detected in large spheroids.
Marker B224C4P2 was located 3.1 kb from Ca_Sb07g023840, and both markers were separated by a minimum of one and a maximum of three recombination events in the Tift 23DB × ICMP 451 map (Ca_Sb07g023840 was scored as a dominant marker; hence, not all recombination events could be identified).
Both markers were assessed with a DAX 96, Technicon Instruments Corporation, Tarrytown, NY, USA, 1993 1996.
Both markers are associated with a similar response as mentioned in [ 1]. Figure 4 depicts the grand averages.
Restriction digestions for both markers were conducted in a 10-μl volume with 1× buffer (New England Biolabs), 1 μL PCR product, 5 10 units of enzyme (New England Biolabs), and 100 μg/ml Bovine Serum Albumin (when required).
The highest rate of lymph node- and distant metastases could be obtained in the group of CC with combined scores of high SOX2 and high β-catenin expression, whereas tumours with low or absent expression of both markers were associated with a reduced risk for loco-regional and distant spread.
Both markers were identified using an immunoluminometric assay (sandwich test).
The combined PSP/PCT score had a NPV of 100% if both markers were below cutoff and a PPV of 71% if both were positive.
The best discrimination was achieved when both markers were used jointly, yielding a ROC AUC of 0.84 (95% CI 0.76, 0.93).
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CEO of Professional Science Editing for Scientists @ prosciediting.com