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Sal09 and Sal10 were similar in both map length and number of markers.
Both map length and marker density across chromosomes were moderately correlated (r = 0.56, Additional file 5).
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The combined map had a length of 1190.0 cM, spanning 81% of the I. brevicaulis and I. fulva genome, and calculations of map length and map coverage were similar across both non-integrated maps.
In the reciprocal cross (DI), total map length and average distance were 1462.7cM and 13.9cM, respectively.
Furthermore according to Sekino and Hara [ 56] genome coverage was calculated as the ratio of total map length and estimated map length.
Sal01 and Sal02 had a similar long map length and average marker interval.
Addition of markers to the genetic map will likely increase map length and decrease the latter estimate.
The total length of the Jr-Vv primary SBs was 600.144 Mb (81.5 % of the total physical map length), that of the secondary SBs was 106.351 Mb (14.4 % of the total physical map length), and that of tertiary SBs was 6.920 Mb (0.9 % of the total physical map length) (Table 4 and Additional file 4).
Map length and the number of gene loci per chromosome thus appeared similar in spruce and loblolly pine.
To estimate the corrected linkage map length and coverage, we followed the method of [ 21] and [ 29].
It was determined based on published estimates of total genetic map length and physical genome size as described elsewhere [ 19].
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