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Pluripotency of both lines was tested by embryoid body differentiation, where they gave rise to cell types representative of the three embryonic germ layers (Fig. S1B, C and S2B, C).
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Both lines were tested and the difference between the independent mutants tested as a nested factor using the factor Knockout(Genotype).
Prior to the functional assay, the concentration of siRNA required for silencing, extent of CASP8 knockdown and cisplatin sensitivity (IC 50) in both these cell lines was tested (Additional file 10).
The tumorigenic capacity of both LM-234 cell lines was tested between passages 15 to 20 and between passages 45 to 50.
The antiproliferative activity on HEPG-2 and MCF-7 cancer cell lines was tested.
The cytotoxicity of these compounds against five human cancer cell lines was tested.
Anchorage-independent growth of UT-SCC carcinoma cell lines was tested using the soft-agarose assay.
Invasive potential of three colorectal cancer cell lines was tested.
Tumorigenicity of the cell lines was tested by injecting 2 × 10 cells subcutaneously into athymic mice.
In addition to proliferative responses, the production of IFN-γ by CTL lines was tested.
Both cell lines were tested for mycoplasma contamination using a PCR-based method.
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