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A total of 18,364 clones from both libraries were tested.
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However, only a few examples exist where interactions between extracellular proteins from human and pathogen libraries were tested.
Enriched libraries were tested for enrichment using quantitative PCR.
Other ancient DNA and modern DNA libraries were tested using the same procedure.
Before sequencing, libraries were tested using the BioAnalyzer to assure library quality, in terms of size and primer-dimer depletion.
The four replicate male and female libraries were tested for uniformity using a Spearman's rank order correlation test.
The libraries were tested for the presence and the size of insert by PCR using two primer pairs.
To ensure sufficient adapter ligation, a sample of the libraries were tested with real-time qPCR (primed to the library indexes), and measured against a digital standard curve [ 35].
The differences between the two libraries were tested using the chi-square test and the fisher-exact test, and the log2 ratio was regarded as a threshold to detect miRNAs expression and target genes degradation fold changes.
In total, approximately 19,000 representative compounds from the BIOMOL, Chembridge, Maybridge, MicroSource Discovery, NIH Clinical Collection, Prestwick Chemical Inc. and Sigma-LOPAC commercial libraries were tested using the screen for chemical modulators of longevity.
Additionally, six independent pair wise comparisons between replicated libraries were tested (Table 3); each of these yielded a range of differentially expressed genes, varying from 148 genes (Vienna-7 irradiated pupae vs. non-irradiated pupae) to over four thousand genes (wild adult vs. wild pupae).
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