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Both libraries were screened by hybridization on colonies with probes constituted first by an Abi cox1 cDNA and by cloned PCR products (Supplementary information files Figure S1).
Both libraries were screened in parallel for stabilized Fc-mutants.
To enrich for transcripts expressed primarily in male tissue, both libraries were screened with cDNA generated from conspecific female abdominal tissue, and only non-hybridizing clones were sequenced.
To enrich for transcripts expressed primarily in male tissue, both libraries were screened with cDNA generated from female abdominal tissue and only non-hybridizing clones were sequenced.
Following optimization and establishment of appropriate conditions, both libraries were screened to identify targets for which knockdown enhanced representation in GFP high cells following induction of Xist RNA.
Both libraries were screened for orthologous EST sequences of the five P. caudatum Hsp70-groups using following P. tetraurelia nucleotide sequences (see GenBank accession numbers) as references: CY-A = CR932269 and CR933372; CY-B = CR933371, CR933370 and CR933369; ER-A = CR932268 and CR932267, ER-B = CR932266, MT = AF031366 and XM_001461342.1.
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Both genomic libraries were screened for SSR motifs using the Msatfinder script implemented in PERL ([ 57]; available at http://www.genomics.ceh.ac.uk/msatfinder and all SSRs having repeat motifs of two or greater base pairs and greater than five repeating units were identified.
Both antibody gene libraries were screened with two different panning strategies.
Initially, filters from the Oregon State University library were screened for type I clones using P-labeled cloned probes, and later the PCR super-pools from both the Ohio State University and the Swanson libraries were screened for clones containing additional type I loci and microsatellite loci using specific PCR primers for each locus.
Cosmid libraries were screened by PCR using OneTaq 2× Master Mix with GC buffer (NEB).
Peptide libraries were screened by surface plasmon resonance (SPR) to identify RBD binding epitopes.
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