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* The values are not a simple sum of those for endo- and ecodormancy, because there were common contigs to which reads from both libraries were mapped.
Reads from both libraries were mapped strand-specifically to quantify miRNA and miRNA* expression using Blastn (e-value < 0.0001, 100% identity, S = 1 for miRNA and S = 2 for miRNA*).
To identify novel miRNAs, reads ranging from 20 to 24nt from both libraries were mapped against cassava intergenic regions (Table 1) and the adjacent ± 150nt region was extracted (in total 64,876 regions).
+percentage for non-redundant reads were calculated based on these total reads Reads from both libraries were mapped to the cassava genome (Table 1), resulting in eight million (NI) and seven million (I) reads perfectly mapping to the genome.
To identify conserved miRNAs, reads from both libraries were mapped to a set of known plant miRNAs from miRBase [ 25] and PMRD [ 27]; 56 conserved miRNA families were identified in cassava.
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Reads ranging from 20 to 24nt from both sRNA libraries were mapped strand-specifically against all identified possible targets for all cassava miRNAs to quantify possible transient siRNAs and mRNA fragments generated by miRNA-mediated cleavage (e-value < 0.0001, 100% identity, S = 1 for miRNA and S = 2 for miRNA).
To identify phylogenetically-conserved miRNAs, reads from both small RNA libraries were mapped to the set of all mature Viridiplantae miRNAs obtained from miRBase release 16, September 2010 [ 45] and the complete plant miRNA set obtained from the Plant MiRNA Database PMRD, v. September 2010 [ 22].
A total of 30,423,983 (84.07%) and 32,279,312 (84.41%) clean reads in the two libraries were mapped to the reference genome of rice using bowtie software and allowing a 2-bp mismatch.
Sequence reads from all forty libraries were mapped to exonic EvoFold hairpins.
All sequencing libraries were mapped separately to this assembly.
The libraries were mapped to transposons allowing two mismatches.
More suggestions(15)
both libraries were hybridized
both libraries were tested
both groups were mapped
both libraries were constructed
both studies were mapped
both libraries were run
both libraries were stored
both libraries were searched
both libraries were provided
both libraries were analysed
both libraries were screened
both babies were mapped
both libraries were subjected
both sources were mapped
both libraries were combined
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