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Read length clearly had the most dramatic impact on read mappability, however, and decreases in successful read mapping were strongly correlated with increasing read length for all damage levels and GC contents at both levels of divergence.
In contrast to variant call accuracy, variant call completeness was substantially impacted by coverage depth at both levels of divergence for both SNPs and indels (Table 2, Figure 3).
We observed for both SNP and indel recovery at both levels of divergence that ratios of homozygous to heterozygous variant calls approached their expected values with increasing coverage depth, but closely approached expected values only for SNPs at the highest coverage depth tested (16×).
For indel variant calls, quality filter 1 was the most consistent for variant call success across damage treatments and for both levels of divergence, and either the best-performing quality filter or only slightly less successful in accuracy than the other quality filters.
GC content, read length and damage all had significant impacts on the proportion of read pools able to be mapped back to their reference at both levels of divergence, while coverage depth was not significantly correlated for either low or high divergence read pool mapping (Table 1).
For SNP variant calls, essentially no difference was found in variant call success between quality filters 1-3, while quality filter 4 performed slightly worse (as quantified by variant call accuracy and completeness) across damage treatments and for both levels of divergence.
Similar(54)
For 13 genes we were able to get sequences from both D. atripex and D. phaeopleura, revealing that both exhibit similar levels of divergence to D. ananassae (on average, 20% at synonymous sites and 2% at nonsynonymous sites) and that the choice of outgroup does not bias estimates of interspecific divergence (Table 1).
For comparison, two other groups of related bacteria with similar (i.e., between-strain comparison in Mycoplasma hyopneumoniae; see Figure 3B) or even higher (i.e., between-species comparison in Bacillus; see Figure 3C) levels of divergence both had a higher level of conservation in their chromosomal organization.
The levels of divergence recorded both within and between the species for the four studied loci, are comparable to those that have been estimated for other innate immunity genes of Anopheles [19], [20], [22].
With these data, we compared the families and levels of divergence of units in both regions and interpreted the data in relation to the numbers of rDNA loci and levels of divergence in the intergenic spacer sequences (IGS).
These high levels of divergence are likely to reflect both long periods of isolation between populations and high mutation rates.
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