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Dnmt3L −/− male germ cells (from both knockout lines) have been shown to be hypomethylated at repeats leading to widespread transcriptional reactivation and meiotic failure (Webster et al. 2005; Bourc'his and Bestor 2004).
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These knockout lines have been maintained for more than 10 generations.
Single and double mutants of maize GS1 knockout lines have also been isolated for the cytosolic GS genes ZmGln1-3 and ZmGln1-4, and the mutant plants were also affected in growth and grain yield [ 15].
In this class, single knockouts do not show obvious aberrant phenotypes [ 44], and the generation of double knockout lines has been hampered by the genes' close proximity on chromosome 1.
Phenotypic analysis indicates that tcp23-1 knockout line has an early-flowering phenotype while overexpression lines (OxTCP23 and OxTCP23:eGFP) presents opposite phenotype.
Molecular profiling of the fpgs03g knockout line has generated several new insights into the regulation of folate homeostasis in plants.
This TbAT1 knockout line has been described previously.
The knockout line had slightly larger leaves (5%), but again this difference was not statistically significant in the experimental set-up chosen.
However, we observed sizeable decreases in the rigidity of infected erythrocytes in both knockout lines.
Both knockout lines showed no expression of full length AtCHR23 transcript.
Studies on knockout cell lines have shown that BAX and BAK are functionally redundant.
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