Using both kinetic studies and matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS), we comprehensively examined the 5′-nuclease activity of Taq DNA polymerase on fluorogenic double-stranded probes of varied structures.
It should be noted that both kinetic study on the inhibition of AChE and molecular modeling revealed that compound 8e interacted simultaneously with both the catalytic active site (CAS) and peripheral anionic site (PAS) of AChE.
GF confirmed the kinetic study and contributed to the preparation of the revised manuscript.
Collectively, through a systematic approach, using both kinetic studies with synthetic peptides and recombinant proteins, our data demonstrate that amino acid region 334−335 plays an important role in the expression of factor Va cofactor activity.
Advantages of this mode of operation are pointed out both as regards to kinetic studies and for industrial processing.
As mentioned above, NADP+ ejection has been linked to the last step in the catalytic cycle of both PvdA and SidA by kinetic studies, and the difficulty in deconvoluting the spectra for this oxidative process in SidA hints that something more complex than mere dissociation of NADP+ may occur.
Lima, C. R. et al. Combined kinetic studies and computational analysis on kojic acid analogs as tyrosinase inhibitors.
AGP was involved in the batch kinetic studies and drafted the manuscript.
For these purposes, kinetic studies and adsorption isotherm measurements were carried out.
