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Both kidneys were excised immediately and cut into four equatorial sections; they were then washed twice with cold PBS.
Animals were then injected intravenously with NST-732 (35 mg/kg) for a time period of 2 h and both kidneys were excised, frozen in liquid nitrogen stored at −80°C and further submitted for histological sections.
During the course of reperfusion, animals were injected intravenously with 20 mg/kg of DDC and 4 h later both kidneys were excised, frozen in liquid nitrogen, and stored at −70°C until use.
After the end of reperfusion phase mice were sacrificed using over dosing of anesthesia, blood samples were taken directly from the heart, both kidneys were excised, the right one homogenized then kept in deep freeze at - 80°C for oxidative stress measurement and the left kidney fixed in 10% neutral buffered formalin for histological examination.
Similar(56)
Both kidney were excised, the right one homogenized for oxidative stress parameters (MDA and GSH) measurements and the left kidney fixed in formalin for histological examination.
After two hours, kidneys were excised and prepared for analysis.
Kidneys were excised after which the renal capsule was removed.
The animals were sacrificed after week 20 and liver and kidneys were excised.
At sacrifice liver and kidneys were excised and wet weight recorded.
In some cases hearts and kidneys were excised from previously frozen embryos in phosphate buffered saline (PBS) solution pH 7.4.
After seven days, mice were sacrificed by CO2 inhalation, and kidneys were excised aseptically and homogenized in 10 ml sterile water.
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CEO of Professional Science Editing for Scientists @ prosciediting.com