The positions that affect the binding of ATP (residues Lys214, Glu215, Gly303, Tyr307 and Lys337) and Ca2+ (residues Asp12, Lys19, Gln138 and Asp155) to actin are conserved in both tarantula actin isoforms compared with other species[ 43].
Desensitization of PAR1 signaling was markedly impaired in MEFs lacking both betaarr1 and betaarr2 isoforms compared with wild-type cells.
Dorsal root ganglion and olfactory epithelial neurons both displayed preferential migration on laminin isoforms compared with other ECM proteins, such as collagens and fibronectin (Refs 5, 6).
Although RBPs and TFs both have more isoforms than the general proteome, RBPs have significantly more isoforms compared with TFs.
We identified a much larger number of genes with differentially expressed transcript isoforms compared with genes with differences in overall levels of expression.
Our analysis revealed that the adult mouse brain expresses a distinct combination of LEF1/TCF protein isoforms compared with the embryonic brain or any other adult or developing tissue.
Genes with alternative transcription events on average produce more complex (larger numbers of introns; P < 10−36) and more numerous (P < 10−32) isoforms compared with genes that undergo only AS (supplementary table S5, Supplementary Material online).
As presented in Figure 4A, [S]-GTPγS binding was significantly elevated in membranes from HEK293 cells containing either of the hGPR17 isoforms compared with membranes from empty pcDNA3 vector transfected cells.
For these studies, we evaluated single muscle fibers from each patient and a control muscle, a more accurate and powerful method of determining the expression profiles of MHC isoforms compared with whole-muscle homogenization experiments.
Proteins derived from HEK293 EBNA and HEK293-6E celinesnes had similar glycostructures consisting of more heterogenous and mostly basic isoforms compared with CHO, indicating a lower amount of sialic acids [ 35].
