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HFF and hES2 cells had higher levels of both isoforms compared to most of the cancer cell lines.
Consistent with this, inhibition of MRE11 in K562 cells by inclusion of mirin in the culture medium substantially increased the normally low basal level of topoisomerase II complexes detected by TARDIS assay in this cell line, 100 µM mirin produced a 50% increase in the mean FITC signal for both isoforms compared to the untreated cells (Fig. 6).
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These results were confirmed using another non-tumorigenic cell line from a different species such as MDCK, obtaining similar results despite of the lower level of overexpression of both ChoK isoforms compared to the HEK293T cells.
Expression of cyclin D1 isoforms compared to 18S rRNA was evaluated with the comparative threshold method (Ct).
Also the inhibition of CA II and CA IX and molecular docking reveal brinzolamide to be a more potent inhibitor among the other catalytically active CA isoforms compared to dorzolamide.
Given our knowledge of each muscle's architecture and the multifidus' operating range, we hypothesized that multifidus would have higher elastic modulus with corresponding smaller titin isoforms compared to longissimus or iliocostalis muscles.
In homozygous H1 infants bearing both the p-198A and int2-180A mutations, we observed a 4-fold decrease in the level of placental DC-SIGNR transcripts, disproportionately affecting the expression of membrane-bound isoforms compared to infant noncarriers (P = 0.011).
We have also found that migrating glioma cells express significantly increased levels of V0/V1 isoforms compared to non-migrating cells.
Specifically, CA smelt myotomal muscle will great expression of fast MyHC isoforms compared to the myotomal muscle.
It suggests that those anciently duplicated genes tend to evolve toward having more AS isoforms compared to those non-duplicated genes.
RNA-Seq provides a far more precise measurement of transcript levels and their isoforms compared to other methods such as microarrays.
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