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Therefore, the spectra we report here represent the total contribution from both internalized NPs as well as any NPs still on the cell exterior.
This situation is actually similar to what happens when metal NPs are introduced in vitro or in vivo, where both internalized and cell surface-bound NPs will contribute a plasmonic response upon illumination [53].
Cells treated with non-enzymatic cell dissociation medium display cellular fluorescence from both internalized and membrane-associated peptide.
Such a process of intracellular dehalogenation or degradation, leading to radioactive products that are excreted by the cells, might also explain the time dependent reduction of both internalized activity and total cellular uptake of 125I-labeled CaIX-P1 at 37°C.
These participants explained that these feelings were both internalized and informed by cultural traditions.
This difference was not obvious in our studies because both internalized Y1 YFP receptors and Y1 receptor β-arrestin2 BiFC complexes accumulated in similareceptor β-arrestin2tments.
Similar(49)
Notably, immunoblotting revealed an association of de novo synthesized tTG with both the internalized and recycled proteins in these cells (Fig. 5E).
The internalized receptors (both the LDL receptor-related protein and the LDL receptor) are recycled back to the cell surface.
Certainly Walser was primarily concerned with overcoming his inhibitions about writing by means of the less definitive "pencil method"; and it is equally certain that unconsciously, as Werner Morlang notes, he was seeking to hide, behind the indecipherable characters, "from both public and internalized instances of evaluation," to duck down below the level of language and to obliterate himself.
Salmonella-free leaves were submerged for 5 min in solution with ~105 cfu/ml of the bacteria and washed with water, ethanol and AgNO3 as described above and analyzed for both surface and internalized Salmonella spp. This serves to eliminate the presence of Salmonella as control to assure the presence of no Salmonella spp. in surface after washing.
How, then, does a community facing both external and internalized homophobia make itself stronger?
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com