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Damage on the backside surfaces had a roughened, curvilinear stippling pattern covering > 70% of the surface for both inserts, consistent with motion at the modular interface.
There was evidence of protrusions into screw holes for both inserts, consistent with creep deformation due to weight-bearing loads or removal of material on the surrounding surfaces.
Articular damage patterns were asymmetrically rotated on both inserts, consistent with femoral internal rotation (tibial external rotation) of the right TKA and femoral external rotation (tibial internal rotation) of the left TKA.
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Third, for each individual clone targeted, we normally obtained 1-4 dinsertst inserts, consistent with a recent report that the copy number of Tol2 and piggyBac in HeLa cells ranges between 1-3 and 1-4, respectively [ 34].
We observed a staining pattern in brain tissue (Figure 2A and insert) consistent with a cytoplasmic localization iOPN.
In our case, the backside damage patterns on both PE inserts are consistent with an abrasive mechanism resulting in generation of wear debris and removal of material, to the extent that the alphanumeric labels were generally unreadable.
As expected, for each of the three different r-GV populations, the immunostaining of the incorporated SIVsm derived inserts is consistent with ongoing r-GV breakdown of the individual clusters and concomitant disappearance of detectible, brightly fluorescent particle clusters.
The planned size of the insert was consistent with the implanted insert in 39/49 (79.6%) of patients.
However, the asymmetric articular damage pattern on the right polyethylene insert was consistent with the mismatch of 8° of femoral internal rotation in relation to the tibial component measured on CT scans of the right knee.
The optimal length for integration efficiency and ease of detection was a dsDNA donor with 1 kb homology either side of the inserted sequence, consistent with previous results when the DSB was induced with a zinc finger nuclease (Beumer et al., 2013).
Both are consistent with the set value.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com