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For both insertion lines only wild-type and heterozygous plants were identified, indicating that plants containing an insert in both copies of either RICESLEEPER1 or RICESLEEPER2 might be lethal at a very early stage.
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The presence of unfertilized ovules in both insertion lines prompted an analysis of pollen to determine if that might be contributing to the failure of fertilization.
The previous screening among FRT-combined transposon insertion lines included only dPob/EMC3 among EMC subunits.
Application of mild salt stress increased the mutant allele transmission in both p5cs2 insertion lines, presumably by induction of P5CS1 expression.
The lack of phenotypes could be in part due to the fact that the T-DNA insertions in those lines only lead to an incomplete loss of SRF function.
After adaptation to controlled environmental conditions, the Rap2.4a-KO T-DNA-insertion lines showed only subtile phenotypes: The chlorophyll levels were slightly decreased and the leaf blades were 8%% larger (Fig. 6A and 6B).
The use of P-element insertion lines identified CG14891 gene disruption as the only one causing a significant decrease in second male paternity success relative to wild-type and ebony tester males.
The Southern blot indicated for most of the analyzed lines only single T-DNA insertions, including the stable control lines (ICE 1.1.1 and PNA 8.6).
Both mop mutants are derived from a pool of T-DNA insertion lines (Sieberer et al., 2003).
We analyzed several transposon insertion lines for expression of Roc2 and identified two mutant alleles, both of which are viable.
We found a seed phenotype in two additional T-DNA insertion lines disrupted in At4g32420 (Sand_078665; GABI_785E11) and confirmed through allelism tests that both were allelic to emb104.
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