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The assay solution for both initial and total activity measurements, whose final volume was 1 mL, contained 100 mM Hepes KOH (pH 8.0), 25 mM KHCO3, 20 mM MgCl2, 3.5 mM ATP, 5 mM phosphocretaine, 5 units NAD-glyceraldehyde-3-phosphate dehydrogenase (NAD-GAPDH), 5 units 3-phosphoglyceric phospokinase (PCK), 17.5 units creatine phosphokinase (CPK), 0.25 mM NADH, 0.5 mM RuBP, and 50 μL sample extract.
Both initial and total Rubisco activity in Al-treated leaves decreased to a lesser extent than CO2 assimilation.
Both initial and total Rubisco activity, contents of Chl and total soluble protein in P-deficient leaves decreased to a lesser extent than CO2 assimilation.
Both initial and total ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) activity in Mn-excess leaves decreased to a lesser extent than CO2 assimilation.
Both initial and total Rubisco activity was higher in -Al leaves than in +Al ones except that there was no significant difference between the two under 25 μM B (Fig. 4D and 4E).
This inference was also supported by the results that both initial and total Rubisco activity in Mn-excess decreased to a lesser extent (Fig. 1D-G) than CO2 assimilation (Fig. 1A).
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In the other one study, we found that both the initial and total Rubisco activity was lower in 1.2 mM Al + 10 μM B treated leaves than in 0 mM Al + 10 μM B treated ones under 50 or 100 μM P, whereas there was no difference between the two under 250 or 500 μM P (data not shown).
Initial and total amounts of urinary gonadotrophins per cycle were approximately 30% higher compared with rFSH for IUI and IVF.
The tensile strength, and initial and total fracture energy, however, were not found to be size dependent.
Unpaired t-tests were used to compare the baseline (pre-treatment) characteristics of the group of patients who completed both initial and follow-up outcome measures and the initial total group.
We also found correlations between both markers' initial levels and total cholesterol and hsCRP, with opposite direction of mentioned correlations for OPG and TRAIL.
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both initial and 1-year
both selective and total
both initial and modified
both initial and long-term
both initial and terminal
both hagfish and total
both initial and residual
both initial and final
both initial and post-thinning
both remarkable and total
both joyous and total
both initial and relapse
both postpaid and total
both initial and ongoing
both intact and total
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