H3K36me3 showed the same profiles in both the induced and uninduced conditions.
H3K4me2 is localized to the 5' region of this gene, in both the induced and uninduced states, and in cells null for STAT1 as well.
Both of the induced and uninduced SOD were detected by western blot, but the intensity of the induced SOD was higher than the uninduced SOD.
Salmonella typhimurium gene mutation (Ames) tests of 1,3-butadiene using strains TA1535, TA97, TA98, and TA100 and employing rat, mouse, and human liver S9 metabolic systems were barely 2-fold above background only in strain TA1535 at 30%1,3-butadiene1,3-butadiene induced and uninduced rat S9 and mouse S9 (uninduced).
We did not observe changes in β-actin mRNA distribution along the gradient between induced and uninduced cells (Fig. 3A).
Cellular RNA was reverse transcribed into cDNA with Alexa-647 and Alexa-555 fluorescent dyes used to label the cDNA from induced and uninduced cells, respectively.
For the point mutants, PcrV, PopB, and PopD were similarly expressed and retained within bacterial cells at the level of the ΔV+pcrV strain under induced and uninduced conditions (bacteria, Fig. 7C).
An initial survey of 5,000 plaques from the original differential screen for early responses to signals from a graft of Hensen's node [35] identified 49 clones showing differential expression between the induced and uninduced libraries.
The difference in protein levels between induced and uninduced samples was a factor 2 5 fold (all protein levels were determined from immunoblot by densitometry using ImageJ, data not shown).
Metabolic labeling was performed on induced and uninduced T1V1 and T1L1 alongside 143B.206 ρ+ parental cells.
