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Consistent with these reports, we found that valine substitutions a 441 and 442 had severe consequences: while Thr442Val displayed a non-expressing phenotype (Zheng and Sigworth, 1997), the Thr441Val mutation resulted in voltage-dependent currents both in the inward and the outward direction, as well as reduced potassium selectivity, suggesting a significant perturbation of the local structure.
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Crystal structures of the mammalian GLUT5, both in the inward-facing and outward-facing conformations, were previously determined37.
In the two crystal structures, the final refined structural models contained the intact peptide chains of residues 10 400 and 14 400, respectively, and were both in the inward-facing conformation consistent with the previously reported Dxc-ecMdfA structure (Heng et al. 2015).
In contrast, the double mutant R228C-C136A totally suppressed the ability of Cd2+ and of Cu-Phen to activate the current both in the outward and inward direction and to induce an instantaneous component (figure 7A and B and Table 1).
The experimental data were interpreted in the context of molecular models of DAT in both the inward- and outward-facing conformations.
Most of the Poynting fluxes are anti-sunward with a small inward component both in the dawn and dusk sectors.
Finally, the Ct site is observed in both the inward- and outward-facing states, suggesting that the apo-like transport domain could carry the ion across the membrane.
Trust in the Inward Light is the distinctive theme of Quakerism.
These data suggest that the mutant DIII voltage-sensing domain may be stabilized in the inward conformation.
Uncleanness had to be dealt with in the inward, spiritual personality of those afflicted.
In the inward TS type, the third and fourth spot arrays are elongated toward the discal spot.
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