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We have recently reported that activation of the Ras-MAPK pathway by Src in quail myogenic cells is largely responsible for inhibition of differentiation both in myoblasts and in post-mitotic myocytes [16].
In this case, given the expression of lb both in myoblasts and during morphogenesis, behavioural assays additionally allow the use of genetics to isolate interacting genes as well as alleles that may allow the dissection of the roles of lb between muscle specification and morphogenesis.
In contrast, the expression of β2-syntrophin occurs both in myoblasts and myotubes without significant changes in the level (Figure 6A).
It is noteworthy that, beyond control of cell cycle, p27 may regulate several other functions both in myoblasts and in other cell types, such as cadherin-mediated cell-cell adhesion [4], tumor progression, apoptosis and cytoskeletal dynamics [38].
Both in myoblasts and in myotubes, levels of the cell cycle inhibitor p27 inversely correlated with miR-221 and miR-222 expression, and indeed we show that p27 mRNA is a direct target of these miRNAs in myogenic cells.
Control pGL3 reporter vector or pGL3 containing the short portion (s-UTR) or the full length (UTR) p27-3'UTR were transfected in QMb-ts myoblasts and luciferase activity was measured both in myoblasts and in myotubes (Fig. 6A).
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Also, we observed a possible interaction of MVI with talin as well as a correlation between MVI and talin expression levels both in undifferentiated myoblasts and mature myotubes.
Interestingly, depletion of Suz12 resulted in the loss of both Ezh1 and Ezh2 proteins in myoblasts and myotubes.
Furthermore, morphological differentiation abnormalities were reported in myoblasts and mesangioblasts isolated from FSHD patients [17], [30].
Dl RFP puncta are visible in myoblasts and myoblast cytonemes.
(B) Polyadenylated H2B1D (H2B1DPa) mRNA fold difference in myoblasts and myotubes.
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