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These findings strongly support that the moving foci both in interphase and during mitosis probably represent dynein complex containing the heavy chain.
Indeed, depletion of CENP-C prevented kinetochore localization of CENP-T/W both in interphase and in mitotis (not shown), whereas depletion of CENP-T/W did not alter CENP-C localization.
In addition, MCAK binds to the plus-ends of growing non-kinetochore MTs both in interphase and mitosis through direct association with EB1 and TIP150 (Honnappa et al. 2009; Jiang et al. 2009; Lee et al. 2008; Moore et al. 2005).
Indeed, basal bodies and centrioles are interchangeable structures; in proliferating cells, the centrioles sit within the centrosome and anchor the pericentriolar material that is required to nucleate microtubules both in interphase and mitosis, while upon exit from the cell cycle and entry into quiescence, the centrioles move to the cell surface and start to act as basal bodies.
Later studies showed that the centrosomal association of macroH2A1 is not restricted to undifferentiated ES cells and is observed in both female and male somatic cells, both in interphase and in mitosis [22], [23].
GFP-tubulin imaging showed that MT nucleation in these strains was again correlated with Mto1 localization, both in interphase and mitosis.
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Ou, H. D. et al. ChromEMT: visualizing 3D chromatin structure and compaction in interphase and mitotic cells.
Also, the cell remained in interphase and did not progress to M phase (Fig. 7, A).
In contrast to disengagement in mitosis, new centriole growth occurs in interphase and is dependent on Cdk2/Cyclin E [4].
Since the missegregation occurs in mitosis, Mis17 might be hyperphosphorylated in interphase and further modulated during mitosis.
Condensin accumulated at induced Hsp genes in interphase and even in mid-mitosis.
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