We have used TAGIT-1 to construct gfp insertions into the B. subtilis minCD operon [13], but when we attempted to use TAGIT-1 to isolate in-frame insertions on E. coli plasmids, we found that both in-frame and out-of-frame TAGIT insertions produced indistinguishable levels of β-galactosidase activity.
The 1500 fusions from normal tissue contribute to the negative set and provide both in-frame and frame-shifted fusions.
PPIs supported by both in-frame and out-of-frame fusion prey clones have occasionally been found, and translational frameshifting has been reported and biochemically confirmed in previous YTH analyses.
In fact, in most species, exon 1A contains two ATG (here named ATG1 and ATG2) that can potentially initiate the translation: both are in frame and exhibit a canonical Kozak sequence, an indication of functionality.
As such, it depends heavily on how issues are selected and framed, and implies the involvement of stakeholders both in issue-framing and design of the assessment, and to help interpret and evaluate the results.
The term includes both structural timberwork in framing and items such as doors, windows, and staircases.
Global regulation of this type, involving both an in-frame isoform and an NMD-sensitive transcript, has been described for serum response factor (SRF) [ 40].
Conceived as a doubles tennis partner for his brother Wario, Mario's evil doppelgänger, he was thin in both frame and character development.
The expression construct contained both an in-frame FLAG tag and an in-frame hexahistidine (His) tag at C-terminus of the Ac-DAF-16 DBD, as confirmed by DNA sequencing (Nevada Genomics Center, Reno, NV).
Both deletions result in frame shift and early termination of protein translation.
To eliminate expression arising from transcripts containing the alternative internal exons in unc-62, a single base pair was inserted within that exon to create both an in frame stop codon and a translational frame shift.
