Sentence examples for both immunostaining and quantification from inspiring English sources
Cell-specific markers were selected and utilized in both immunostaining and quantification with RT-PCR.
They were scanned using [18F]FEOBV PET, then sacrificed, and their brain tissues collected for immunostaining and quantification of cholinergic denervation using optical density (OD).
Immunostaining and quantification of inflammatory cells will be performed according to standard procedures [ 4, 23, 25, 26].
(F, G ) Immunostaining and quantification of Phospho-H3 (green) in EGL of wild type and Nrp1/2 DKO cerebellum at P7. Scale, 100 μm.
Immunostaining and quantification of cleaved caspase 3 in the TEBs was done to evaluate whether Cdc42 overexpression might be increasing MEC survival, and again, no differences in apoptosis rates were detected between the Cdc42-overexpressing and control mammary glands.
Endothelial-specificity of overexpression was confirmed by several approaches including immunostaining and the quantification of Nox2 levels in isolated endothelial cells and in aorta with or without endothelium.
Immunostaining and satellite cell quantification were done as we previously described [9], [71].
Subsequently, the undifferentiated and differentiated SH-SY5Y cell lines were immunostained for phosphorylated NFs, and quantification of the mean fluorescence intensity confirmed the significant increase in phosphorylated NFs in the mutant HSPB1-S135F HSPB1-P182L182linesl lines (Fig. 5d g).
Only TBS-1 was significantly increased within the adult virgin mammary glands of the p190B+/-Neu mice, and quantification of immunostaining for TBS-1 confirmed this increase.
Both intensity of immunostaining and percentage of immunopositive area were recorded at 40 × magnification.
Immunostaining and followed by Nanog+ colonies quantification further proved that almost every Oct4-GFP+ colony is also Nanog+ at that stage (Supplementary Figure S6c) and miR-106b can enhance formation of both colonies (Supplementary Figure S6d and e).
