Cell-specific markers were selected and utilized in both immunostaining and quantification with RT-PCR.
Both hSRBC immunostaining and methylation results did not correlate with clinicopathological characteristics of these patients.
Both isoforms contribute to a more migratory phenotype, as shown by immunostaining, cell migration, and KD experiments.
Both intensity of immunostaining and percentage of immunopositive area were recorded at 40 × magnification.
Anti-human specific nuclear antigen immunostaining was used to trace the survival and migration pattern of the HUMSCs.
In this study, the phenotypic expressions of normal human dermal fibroblasts (NHDFs) on collagen-containing nanofibrous matrices with either isotropic (i.e., fibers collected randomly with no alignment) or anisotropic (i.e., fibers collected with alignment) fiber organizations were studied by immunostaining, migration assay and molecular analyses.
Images of individual dye immunostaining and overlays of both dyes were obtained using ImageJ [40].
Molecular analyses were performed in both experiments, including cell proliferation assessment (Ki-67 immunostaining), and Phosphoinositide 3-kinase/mammalian target of rapamycin target expression studies (p4E-BP1 immunostaining), which were taken as gold standards for therapeutic assessment in the present work.
In 10%% of the regions, both internal and international migration contributed to a negative net migration.
Covering both domestic and international migration, especially in the African context, his works relate to trends of migration, migrants' investments, return migration, transnational families, integration and transnationalism..
