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Both hemispheres of each mouse brain were employed, one for histological plaque quantification, the other for biochemical measurement of insoluble Aβ.
The number of calbindin+ve neurons in both hemispheres of each mouse was estimated using the principles of the fractionator method [ 23].
Data were averaged from both hemispheres of each animal and then mean and standard error of the mean (SEM) calculated from three animals per age group.
Volumes (V) were calculated as V = ΣA · i · d, according to Cavalieri´s principle, with A representing the sum of areas from both hemispheres of each section, i the interval between the sections, and d the section thickness, respectively.
We functionally identified each of these regions in both hemispheres of each of 14 human participants and examined their responses to 2D planar motion, 3D flow, trajectory-matched random motion, and hemifield stimulation while subjects performed a central distractor task.
To compare the present results with data in the literature, we performed a meta-analysis in the line of that published by Penhune in 1996 and pooled eight previous studies of HG anatomy that included both hemispheres of each individual (total of 164 brains, Table 4).
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Cells exhibiting a nuclear DAB signal were counted in each of three 1.0-mm-square confocal microscope fields, in each of three spaced coronal sections through the neostriatum in both hemispheres of 6 mice from each treatment group.
The injections were always done into both hemispheres of the brain, two sites on each hemisphere (Fig. 1B).
Since each eye sends some of its nerve impulses to both hemispheres of the cat's brain while each paw only directs impulses to the hemisphere of the brain on the same side of the animal's body, this procedure feeds the sensory information to just one hemisphere.
We drew lesion damage onto ten standardized sections of LEC (ranging from −7.66 to −4.42 mm from Bregma, using Scion Image (v4.0.3.2) and calculated the total area of damage in pixels across both hemispheres for each of the subregions of LEC (VIE, DIE, DLE, and AE) and the subregions combined (the whole LEC).
In total each brain region was analyzed in eight independent samples, spanning both hemispheres of both brains, with two independent sampling sites per hemisphere (treated as biological replicates).
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