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They also share an average of 89% sequence identity with the MaRGA08 probe from M. acuminata Calcutta 4. On both haplotypes, only one complete RGA is predicted on the direct strand (RGA08G-1 and RGA08G-2, Figure 2): it faces an RGA pseudogene on the reverse strand in a tail-to-tail structure (RGA08H-1 and RGA08H-2) with less than 300 bp between stop codons.

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In the populations that had two haplotypes, only one haplotype was found in each nest.

From the five estimated haplotypes, only one showed a nominally significant association (P = 0.019; Table  2).

While between Dzanga Sangha (n = 54; 11 unique haplotypes) and Lope (n = 17; six unique haplotypes), only one haplotype was shared between localities.

Interestingly, 8.1 ancestral haplotype only one, rather than two or more C4 genes and is therefore associated with a relative C4 deficiency.

Besides the haplotypes having only one sequence, some haplotypes were characterized geographically.

The fact that the two most abundant maternal haplotypes, but only one of the low-frequency haplotypes, were identified in the infant suggests that colonization involved a small maternal inoculum, one in which most low-frequency alleles were not represented.

In the haplotype analysis, only one (the CGGCT haplotype) of the three haplotypes carrying the 181946C allele was associated with a significantly increased risk of lung cancer in comparison to the CGGTT haplotype with the 181946T allele.

In those cases where paired homokaryons carrying different haplotypes at only one of the two loci, resulting heterokaryons would be expected to carry either both haplotypes of MIP or both of RCB.

In addition, the BAF at obligate heterozygous sites in the proband (parents homozygous for different alleles) was always skewed towards a greater contribution from the inherited paternal allele, suggesting a second paternal haplotype, and only one maternal haplotype.

For example, the Pik locus harbors multiple Pik alleles activating resistance to rice blast by recognizing different AvrPik haplotypes containing only one to three amino acid changes from one another (Yoshida et al. 2009; Kanzaki et al. 2012; Wu et al. 2014).

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