The hind paws of the rats in different groups were dissected, fixed in 10% neutral buffered formalin for 48 h, and decalcified in 10% (w/v) ethylenediaminetetraacetic acid disodium salt (EDTA-2Na) until the bones were pliable.
One N9 group and one control group were dissected at 10 minutes, and additional N9 groups were dissected 2, 8, 12, 18, and 24 hours post-exposure.
The lungs of the mice in the control groups were dissected at the matching age.
Snails assigned to the time zero groups were dissected within two hours after field collection and digestive glands were preserved in RNA Later® (Ambion, Austin, TX) and stored at -80°C.
Mouse spinal cord samples from different groups were dissected after ˜20 days of treatment, fixed in 4% paraformaldehyde overnight at 4°C, cryoprotected in 30% sucrose, and frozen at −80°C for future experiments.
Another subset of recently-mated females from the four treatment groups were dissected after 48 hr, in order to estimate the rate of female insemination for each experimental group and compare the activity and amount of the sperm contained in their spermathecae.
Debrided (n = 24) and intact rat corneas (n = 24) from the four treatment groups were dissected and trephined (3 mm) at 48 hours and immediately cultivated in a 24-well culture dish with 500 μl of serum-free medium (MEM + minimal essential amino acid + antibiotic/antimycotic) at 37°C, 5% CO2 atmosphere for 72 hours.
Even as new theories about pain were emerging, anti-abortion groups were dissecting abortion-related opinions by Justice Kennedy, especially his 2007 opinion upholding a federal ban on a particular procedure that critics call partial-birth abortion.
The SVZ from individual animals from all 3 different groups was dissected out after sacrificing.
The SVZ from individual animals from all 3 different groups was dissected out and processed for protein isolation [3].
