Purple artichokes have long been part of the gene pool, both globe-shaped ones and the pointy kind, which have been easier to find.
The early explorers, both globe-trotters and narrators -- Columbus among them -- gave open support to this kind of thinking.
Reverse transcription from both globe artichoke and cardoon total RNA was achieved using poly dT primer and M-MuLV RNaseH-RT (Finnzymes, Finland), following the manufacturer's instructions.
Hydroxycinnamoyl-CoA:quinate hydroxycinnamoyltransferase (HQT) encoding genes have been isolated from both globe artichoke and cultivated cardoon (GenBank accessions DQ915589 and DQ915590, respectively) using CODEHOP and PCR-RACE.
Extracts from both globe artichoke and cultivated cardoon have exhibited hepatoprotective, anticarcinogenic, antioxidative and antibacterial qualities, and even an inhibition of cholesterol biosynthesis and LDL oxidation [ 3- 6].
To obtain the full length sequence, specific primers based on both, globe artichoke and cardoon, partial cDNA sequences, were designed for 3'- and 5'-end amplification as described in Comino et al., [ 32].
CODEHOP [ 37] was used to target conserved acyltransferases in globe artichoke (see arrows in Fig. 2), resulting in the amplification of an incomplete internal acyltransferase-like sequence, which was extended in both globe artichoke and cultivated cardoon via a RACE strategy.
The same year, both the Golden Globes and the Screen Actors Guild nominated DiCaprio twice in the Best Actor category for both of his 2006 features, and in addition, DiCaprio earned his third Academy Award nomination for Blood Diamond.
Slumdog Millionaire won the top prize at both the Globes and the Baftas.
And yet Lincoln seems to have been the great glass-jawed heavyweight of this year's award, flattering to deceive at both the Globes and the Baftas.
