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Additional mice of both genotypes were injected and survival was documented for 14 days.
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Adult flies of the indicated genotypes were injected with ∼50 nL and monitored daily for lethality or were collected for immunoblot analysis or viral titers performed as above [44].
The ears of mice with the indicated genotypes were injected intradermally every other day for different time periods with 30 µl 1×PBS alone or containing either 1.5 µg PL and/or 30 ng recombinant murine MCP-1 (R&D).
Adult mice (aged 10 weeks, body weight ≥20 g) of the indicated Ndr genotypes were injected intraperitoneally with 7.4 mg/kg body weight AOM (Sigma) on day 1.
Anesthetized mice (5 females and 10 males per genotype) were injected 0.2-ml of labeled hHDL3 (∼180 µg hHDL3 protein, 1.7 µCi 125I and 0.5 µCi 3H) via the external jugular vein [22], [23].
Nine mice of each genotype were injected with 200000 cells.
To assess whether the increased number of DCX-positive cells was a result of increased proliferation of a progenitor cell population within the SGZ or increased survival of neuronal progenitor cells, mice (n=5 per genotype) were injected with 50 mg/kg 5-bromo-2-deoxyuridine (BrdU) daily on 4 consecutive days.
Furthermore, the lipid and glucose profiles did not reveal any differences between mice of the same genotype that were injected with P. g or FSL-1 (supplemental data: Table S1 and Table S2).
For zygotes at 2-cell stage, both cells were injected.
Both tracers were injected as 3% suspensions in distilled water.
Both drugs were injected in a fixed volume (0.04 mg/100 mg).
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