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To evaluate the inflammatory infiltrate within the lungs, immunohistochemical analysis was done in sections from bleomycin-treated mice of both genotypes using an anti-myeloperoxidase antibody (Thermo Fisher Scientific, USA).
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In our study, we isolated 414 bacterial strains from surface sterilized roots and leaves of both genotypes using a culture-dependent approach.
To identify commonly cold-induced genes within the OsDREB1 regulon, we examined cis-regulatory elements in the 1-kb upstream regions of 991 commonly induced genes in both genotypes using the DRE core motif A/GCCGAC [ 29].
The DNA samples were genotyped using an Illumina HiSeq.
Two hundred seventy nine SNPs, representing 131 genes, were genotyped using an Illumina Infinium iSelect BeadChip as part of the Collaborative Oncological Gene-environment Study (Collaborative Oncological Gene-environment
KIR genes were genotyped using an in-house PCR-SSP.
DH lines from both crosses were genotyped using a 90K SNP chip developed using gene-specific SNPs, which provides a dense coverage of the wheat genome (Wang et al. 2014).
The snails were genotyped using a set of RAD-seq-derived loci that flank the supergene, and instances of recombination tested by comparing inferred supergene genotype against RAD-marker genotype.
The offspring were then genotyped using a set of linked RAD-seq loci that flank either side of the supergene (Richards et al. 2013), and instances of recombination confirmed or refuted by comparing inferred supergene genotype against RAD-marker genotype.
From 14,140 individuals, 2225 were selected for genotyping using a case-cohort design.
A panel of ancestry informative markers (AIM panel with 91 SNPs) was genotyped using a qualified GoldenGate genotyping method.
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