There was no significant difference between genotypes in basal (morning) plasma ACTH levels in either LF-fed (data not shown) or HF-fed mice (GR+/+ vs. GRβ geo /+: 38±8 and 19±7 pg/ml, respectively), consistent with the similar basal plasma corticosterone levels in the latter.
Urine flow rate did not differ between the genotypes in this basal state.
The electric foot-shock used as aversive stimulus was found to produce similar basal reactivity in both genotypes as evaluated, in a preliminary experiment, by the changes of compartment, locomotor activity, jumps and vocalizations (data not shown).
There were no genotype-specific changes in basal spine density at either age (Figure 1B).
On the basis of comparable Sqstm1 mRNA levels between these two genotypes, both basal and post-ligation, and sustained accumulation of SQSTM1 protein in ligated SMGs of Atg5 KO mice, we conclude that duct ligation induced autophagy in SMGs.
In the basal state, the skeletal muscle (P Ser-Akt signal was equivalent in both genotyP Ser-Aktwasignalented 1.8±0.2-fold by insulin in AKAP150ΔPIX samples relative to wascontrols.
In cases where a particular transcript reacted in the same way in both genotypes, the total transcript count before and after inoculation was compared, allowing the identification of basal genotypic differences between the two genotypes.
Also, the ROS was significantly increased in both genotypes when sprayed with NAA, particularly in HTS.
The skin of K5-Ets1 BT mice and the skin of Inv-Ets1 BT mice demonstrate overlapping phenotypes in that expression of cornified envelope genes is impaired, while expression of the basal markers is expanded in both genotypes of mice ((Nagarajan et al., 2009; Nagarajan et al., 2010) and this report).
While iNOS KO mice had a decreased basal ejection fraction, LPS increased ejection fraction in a parallel fashion in both genotypes (Fig. 9e).
