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The UCSC Blat software [ 17] was used to align contigs to both genome and transcriptome references.
Next generation sequencing (NGS) allows fast and massive production of both genome and transcriptome sequence datasets.
SNP accuracies of 98.0% and 91.8% were confirmed, indicating the reliability of both genome and transcriptome data, respectively.
TopHat2 [ 17], using Bowtie2 [ 24], can map reads against both genome and transcriptome (when an annotation is provided).
The emergence of MPS techniques has profoundly transformed the landscape of both genome and transcriptome sequencing [ 11].
This leverages the advantages of both genome and transcriptome mappings as well as combining the speed of Bowtie with the sensitivity and flexibility of Blat.
Similar(47)
Sequence reads, including both genome and transcriptomes, have been deposited into the NCBI sequence read archive (accessions SRA115225).
Sequence census methods such as ChIP-Seq and RNA-Seq are becoming powerful and quantitative approaches to analyse the structures and functions of both genomes and transcriptomes at maximal resolution.
That this pattern of loss is present in three genomes, one of which is the well-sequenced (both genomes and transcriptomes) Nasonia genome, implies that loss of the complex in this group is not a sequencing error, but that Chalcid wasps have lost their E spl -C.
The ability to obtain both the genome and transcriptome sequences of both parental species involved in an interspecies cross will allow unequivocal assessment of the expression of every allele, from either parent, within individual F1 or backcross hybrid progeny.
In addition to the SAL1 gene, several other genes known to be involved in abiotic stress tolerance have been identified as potential targets for improvement by observing changes in the number of family members both in the genome and transcriptome constructed separately from the control, drought and waterlogged RNASeq samples.
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