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Candidates for both genes were identified: ribC as a putative RNA pseudouridylate synthase and leuA as a putative α-isopropylmalate synthase (α-IPMS).
Both genes were identified following high-density mapping at the chromosome 16 locus for SLI (37).
In a cancer-free population, mutations in both genes were identified (15 out of 92) (Keohavong et al, 2005).
Second, both genes were identified as differentially expressed in embryonic glia based on microarray transcriptome profiling (U.G., unpublished).
The double mutant csgl1 + 3 carrying both genes were identified from the above-mentioned F2 population with gene-specific molecular markers (see Table S1 for primer sequence information).
Interactions annotated "Negative Genetic," "Synthetic Growth Defect," or "Synthetic Lethality" were included in this list if both genes were identified as nonessential in our data.
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Because viral culture for HMPV was unavailable, specimens were designated "true" positives if the same amplification product for both genes was identified on repeat testing of the sample extract.
Genes like Nurr1 and En1 were identified with increasing relative temporal expression, hinting increasingly critical roles during mdDA development, as is known from transgenic ablation models where both genes are identified as critical for mdDA development (Jacobs et al., 2009a; Alves dos Santos and Smidt, 2011).
Both AOS genes were identified based on the sequence similarity towards plant Cyp74-enzymes in an EST-library described in [ 18].
Both of these genes were identified by previous screens for inhibitors of phase 2 detoxification gene expression, confirming that our screening conditions were effective at identifying regulators of gcs-1p::gfp [ 13, 17].
To obtain a profile taking into account the expression sets of both tumour types, significantly regulated genes were identified independently for both groups.
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