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Transcriptional analysis of the two genes showed constitutive expression, where both genes were highly expressed in blood under normal physiological conditions.
Here, we report the characterization of two JA-responsive AP2 family transcription factors AaERF1 and AaERF2 from A. annua L. Both genes were highly expressed in inflorescences and strongly induced by JA.
Extending these experiments to PCa and other cell lines revealed that both genes were highly expressed, specifically in the PCa cell line LNCaP (Fig. 4B).
Indeed we could confirm an up-regulation of BMP4 and both genes were highly up-regulated upon OCT4 knockdown in ES and EC cells [6], [9].
Both genes were highly similar at the amino acid level and widely expressed in the body.
We first extracted gene pairs in which both genes were highly expressed or both genes were lowly expressed and tested for differences in Red1 signals.
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Furthermore, tissue-specific expression analysis of OsNCED3 and OsNCED4 genes using RiceXPro database revealed that both genes are highly expressed during lemma and palea development (Additional file 3).
Interestingly both genes are highly expressed in undifferentiated ES and EC cells and their expression declines upon differentiation.
Kinase activation of CDC7 is dependent on expression of DBF4, and both genes are highly expressed in cancer [ 55].
Both genes are highly transcribed from the early embryo until the adult male, including in the testes.
Indeed we found that ENV1 negatively regulates transcription of both gnb1 and gng1, the transcription patterns of both genes being highly similar in the Δ env1 mutant.
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