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To determine the location of the catalytic domain and its contribution to substrate specificity, deletion mutants of both genes were generated.
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In order to uncover the role of Ezh1 in zebrafish development, lines harboring mutations in both ezh1 and ezh2 genes were generated.
Genealogies of individual genes were generated using the same approach.
It appears that 395 PK genes were generated by tandem duplication (Fig. 2; Supplementary Table S5).
Thus, two codon-optimized PfAQP genes were generated.
Differentially expressed genes were generated with Cuffdiff (v1.3.0) with false discovery rate value < 0.05 and filtered by corresponding threshold.
Chromosomal DNA deletions of single genes were generated using 3-step PCR products containing flanking regions 400 600 nucleotides (nt) in length that are homologous to the target sequence.
A large number of rodent carboxylesterase genes were generated from tandem gene duplication.
These fused genes were generated by overlap extension PCR, and the linker sequence between them was 5′-CTGGACAGCACC-3′.
Stable strains expressing each of the genes were generated and cultured in the absence or presence of selenocystamine increasing concentrations.
Primer sequences specific to 12 of target genes were generated with Beacon Designer software (Bio-Rad).
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