Sentence examples for both genes using a from inspiring English sources
Successful incorporation and expression of both genes using a linear purified DNA fragment, containing exclusively rearranged lciA, mutated pds and nabI* genes of H. pluvialis, represents to our knowledge the first functional self-cloning approach in microalgae, a breakthrough that can facilitate the widespread use of genetically altered microalgae.
To identify recombinants, analysis was performed on the gag, env, and concatenated sequences (where samples were amplified on both genes), using a combination of Recombinant Identification Program, RIP 3.0 from the Los Alamos HIV database and SimPlot version 3.5.1 [75].
It is therefore possible to disrupt both genes using a single DNA construct, as has been shown for other paralogous genes in rodent malaria [18], [52] and for adjacent genes encoding aspartatic proteases in P. falciparum [53], [54].
Secondary antibody incubation was performed for 2 hours at room temperature for both genes using a 1 : 1000 diluted peroxidase IgG conjugated anti-mouse secondary antibody (Kirkegaard and Perry Laboratories, Inc .. Specific bands were detected using the ECL Western Blotting Substrates Thermo Scientificc).
As such, primer sets now exist for all eleven genes of common human RVs, allowing for the identification of reassortant strains with mixed constellations of both genotype 1 and 2 genes using a rapid and economical RT-PCR/PCR method.
Our follow-up evaluation of the disease association of both DEPgenes and the additionally recruited genes using a major GWAS dataset for depression found that these genes tended to have small P-values (i.e., at the nominal significance level).
The results were consistent in all these centrality measures; hub genes using a combined network were more representative than hub genes using a single data source network.
We classified the identified genes using a similar method previously described (Kang et al. 2016).
Jennifer Doudna, co-inventor of CRISPR Cas9 technology, or the ability to program genes using a special enzyme, spoke about the promises of this technology onstage at SXSW this afternoon.
AUGUSTUS was run using the following parameters: training data S. cerevisiae strain S288c, gene prediction on both strands, and predict gene using a complete gene model.
In addition, recombination detection was performed on both genes by using a genetic algorithm approach looking at topological incongruence (GARD) [ 58] as implemented in the http://datamonkey.org[ 59] server.
