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Both deletion mutants were non-viable in MARC-145 cells and porcine alveolar macrophages, indicating that both genes are essential for virus replication.
Using two different approaches, promoter replacement, and a novel system of inducible protein degradation, leading to inducible expression of \(clpP1\) and \(clpP2\), we demonstrate that both genes are essential for growth and that a marked depletion of either one results in rapid bacterial death.
Both genes are essential, and the loss of either results in large unbudded multi-nucleate cells, indicative of an inability to undergo polarized growth [7], [8].
Both genes are essential in maintaining the DN3 checkpoint status, when the cell fate declines either to be αβ- or γδ-T cells [34] [36].
If detailed genetic manipulations of individual genes formally show that both genes are essential, chemical genetic approaches based on the present enzymatic studies will follow.
Sonic hedgehog secreted from the floor plate triggers the expression of Mash1 and GATA2 in progenitor cells in the ventricular zone of hindbrain [6], and both genes are essential for the development of 5-HTergic neurons [7], [8].
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An intraoperonic gene pair was considered essential (EE) if both genes were essential and nonessential (NN) when both genes were nonessential.
BRA was necessary for and preceded CDX2 expression; both genes were essential for expression not only of mesodermal genes but also of trophoblast-associated genes.
It was also shown that both tbp genes are essential for iron acquisition from transferrin [ 59], and another recent study showed that a tonB1 mutant cannot use porcine transferrin, but is not attenuated in vivo [ 18].
The ESSL inconsistencies refer to cases where the model predicts that one or both of the genes are essential even though the experimental results show that they form a SL.
All these observations indicate that both CCTα and CCTδ genes are essential.
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