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These regions are presented at both gene and transcript levels.
FPKM (Fragments per kilobase of exon per million fragments mapped) values represent expression level of both gene and transcript.
To ensure the quality of the data, we only use the cDNA sequences of protein coding genes with both RefSeq mRNA ID and the known status of both gene and transcript.
Furthermore, the SE showed higher seasonal differentiation in both gene and transcript expression relative to either VMN or the surrounding hindbrain (Figs. 2, 3), suggesting that at the level of hair cells and motoneurons, seasonal variation in hearing sensitivity requires greater transcriptional changes than the vocal motor system.
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Cuffdiff performs differential analysis at both gene and transcript-level resolution and controls for both variability across replicates and uncertainty in abundance expression estimates caused by ambiguously mapped reads [ 37].
This pipeline performs a complete analysis to determine and quantify both genes and transcripts expression, exploit the alternative splicing identifying expressed splice junctions, poly(A) sites, cassette exons and other splicing events.
Cufflinks uses the Cuffdiff algorithm to calculate differential expression at both the gene and transcript levels.
To support this refinement, and allow our data to be interrogated on a gene-by-gene basis, at both the gene and transcript level, we have generated dotplots of the raw MMBGX output data (as seen in Figures 5A, 6A and 7A) for each gene/transcript and made them available at www.epstein-barrvirus.org.uk.uk
We then utilized the Cuffdiff 2 algorithm [ 70] within Cufflinks 2.0.2 to calculate differential expression at both the gene and transcript levels.
This argues against a simple model where transcription would be shut off for both genes and intronic transcripts would decrease rapidly simply because they are less stable than the mature RNAs.
Meanwhile (RT- qPCR has become the 'gold standaRT- qPCRene and transcript quantification [89].
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