For OS and EC-specific survival, hypothyroidism/levothyroxine was also the only significant factor in both univariable and multivariable analysis, with a significant interaction with surgery.
esg-Gal4 and Myo1A-Gal4 are precursor and EC-specific Gal4 drivers, respectively.
Both full and reduced models are shown.
We evaluated how these two factors influenced EC-specific death and non EC-specific death in a large cohort of EC patients treated with CRT with or without surgery.
EC-specific genes (Cd31 and Flt1), EC-growth factor (Vegf-c), cell cycle regulators (p27 and p53), and pro-apoptotic genes (Caspase3) were not significantly affected in the DKO EC (Figure S2F).
Based on recent findings that pathological angiogenesis and EC dysfunction are accompanied by EC-specific metabolic alterations, targeting EC metabolism is emerging as a novel therapeutic strategy.
Importantly, EC-DKO embryos showed an 8-fold reduction in EC-specific staining for Paxillin and a 4-fold decrease in FAK staining (Figure 6A and 6B, respectively).
It is encoded by intron 7 of the EGFL7 (EGF-like domain 7) gene, which encodes an EC-specific secreted peptide that acts as a chemoattractant and smooth muscle cell migration inhibitor [ 65– 67]; both miR-126 and Egfl7 have a similar EC expression pattern [ 68].
(C) Immunostaining of EC-specific markers (CD31/vWF/CD144/eNOS) in WT, heterozygous (LMNAG608G/+), homozygous (LMNAG608G/G608G) and WRN−/− ECs.
EC-specific deletion of Erk2 in the Erk1−/− background resulted in embryonic lethality at E9.5-E10.5 and the viable EC-DKO E9.5 embryos were smaller than the control littermates.
