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These larval brain atlases have enabled the integration of functional datasets with anatomical markers, in both fixed and live brains.
The method is effective in cell cycle analysis for mammalian cells, both fixed and live, and accurately assesses the impact of a cell cycle mitotic phase blocking agent.
Our laboratory has experience imaging in mammalian cells, yeast cells, Drosophila and Zebrafish embryos and ovaries, bacteria, sections of brain and other tissues, in both fixed and live specimens.
Sensitized emission is useful for measuring FRET signal in both fixed and live cell imaging experiments [35], especially when FRET signals are high.
Here we report the detection of PCNA ubiquitination in both fixed and live cells through the use of spectral imaging to directly monitor FRET between fluorescently tagged ubiquitin and PCNA.
By combining a widely-spaced FRET donor/acceptor pair (CFP and mRFP) with spectral imaging, we have developed a simple method for the visualisation of PCNA monoubiquitination in both fixed and live cells with a single imaging pass.
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Our current ImageXpress Micro configuration permits the visualization of both fixed cells and live cells.
We have confidence in that the SPM techniques could well serve as a promising tool for quantitative studies of both fixed cells and live cells in order to further explore this exciting topic at the convergence of biology and nanotechnology.
Fixed and live visualization of RNAs in Drosophila oocytes and embryos.
Our laboratories are supported by the Otolaryngology Imaging Core (OIC), an advanced imaging center with fluorescence, 2-photon, and four confocal microscopes dedicated for routine and specialized imaging on fixed and live cells or tissue.
Images of fixed and live samples were acquired at room temperature.
More suggestions(15)
both fixed and unfixed
both fixed and unpredictable
both animated and live
both photographic and live
both dead and live
both rehearsal and live
both fixed and spatial
both fixed and mobile-bearing
both fixed and random
both fixed and free
both new and live
both fixed and elastic
both fixed and non-fixed
both fixed and pinned-ended
both fixed and removable
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