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The neurons of both experimental groups showed an excitatory response to electrical stimulation of the dura mater with latencies mostly corresponding to the activation of Aδ-fibers (Fig. 2).
Control joints of all animals in both experimental groups showed no sign of patellar osteophyte formation.
H&E staining of ileal sections from both experimental groups showed that WT and Nod2 −/− mice have normal tissue architecture, and display no signs of inflammation (figure 3A).
Compared with the control group, the right proximal tibia metaphysis of both experimental groups showed significantly higher values of the trabecular separation.
Compared with the control group, the fourth lumbar vertebra of both experimental groups showed significantly higher values of the trabecular separation.
Differences in intratumoral immune response cannot explain this observation, since FACS analysis and IFN-γ-ELISpot of tumors from both experimental groups showed no differences.
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On the contrary, GFAP expression, which was very low on day 4 (data not shown), gradually increased over time in both experimental groups, showing a significantly higher number of BrdU+/GFAP+ cells from 2 weeks on in ephrin-B3−/− mice.
While both the experimental groups showed greater improvements compared to the usual care group, intervention effects tended to maintained longer among couples than individuals.
The recorded data indicated that pregnant females of both control and experimental groups showed a steady increase in body weight during the whole gestation period.
Likewise for the second CWFF, both control and experimental groups showed a significant decrease in mean PD over the last 12 trials compared to the first 12 trials (Control: F 1,78) = 48.6; Experimental: F 1,78) = 32.9; p<.001 in both cases).
However, about 20% of individuals from both the control and the experimental groups showed consistently low Sr Ca ratios irrespective of the salinity, suggesting that the Sr incorporation into the otoliths in these fish was strongly regulated.
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