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A tubing with bacterial heat-moisture exchange filters (Intersurgical "Inter-Therm HMEF" with luer port) at both ends was used to attach the device to the artificial airway, with an oxygen flow of 8 l/min connected to the luer port of the filter close to the MIE device (Fig. 1).
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DNA fragments containing adaptors at both ends were used as a template for amplifying the promoter regions.
Small RNAs with adapters on both ends were used as templates for reverse transcription PCR to create cDNA constructs.
The multiple indexing adapters were ligated to the ends of the dscDNA, then the suitable fragments that had adapter molecules on both ends were used as the templates for PCR amplification, the cDNA libraries were qualified using a 2100 Bioanalyzer (Agilent Technologies, Inc., Santa Clara, CA) and the quality was assessed using an ABI StepOnePlus Real-Time PCR System (ABI, New York, NY, USA).
A fragment of ~260 nucleotides genomic sequence flanking the small RNA at both 5' and 3' ends was used for predicting the secondary structure of the miRNA precursor (stem-loop formations) using the MFOLD program (version 3.2) [ 34, 52].
Rapid amplification of cDNA ends was used to amplify the guppy csf1ra ortholog (Table S1).
The ends were used to justify some truly despicable means.
Rubber or silicon spatulas, short with rounded ends, are used for mixing batter and scraping bowls.
Two types of steel fibres having round cross-sections and hooked ends were used.
These 5' gene ends were used as the estimated reference transcription start sites.
To remedy this, assorted odds and ends were used to reinforce 18th Army.
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