Sentence examples for both ends using a from inspiring English sources

Penetrating intracerebral arterioles from adult C57/BL6 wild-type (WT) mice were isolated at 4 °C, transferred to an inverted microscope and cannulated at both ends using a dual glass micropipette system, wherein intraluminal flow (0.2 μl/min) and pressure (60 mmHg) were maintained.

The U6 promoter fragment was amplified by PCR with introduction of EcoO109I sites at both ends using a pair of PCR primers (Forward: 5'-CAAGGCCCTTTGACGTCAATGGGAGTTTGTTTTGG-3'; Reverse: 5'-TTAGGGCCCGAGCGGATAACAATTTCACACAGGAA-3').

Low-quality bases (Q < 15) were trimmed from both ends using a customised program, and the adapters were trimmed using Cutadapt ver. 1.0 (http://code.google.com/p/cutadapt/) with the parameters '-f fastq -e 0.1 -O 5 -m 20'.

Plasmids were recovered from 20 clones from each of the three donor varieties using the QIAGEN plasmid purification kit (QIAGEN, Hilden Germany), and these were subjected to sequencing from both ends using a BigDye terminator v3.1 ready reaction cycle sequencing kit in combination with an ABI3730x1 sequencer (Applied Biosystems).

Weave the yarn through the stitches of both ends using a whip stitch to join the ends together.

The fiber was sealed on both ends using an arc-fusion splicer to fuse the ends by localized melting.

Multiplexed libraries were sequenced for 36 bp at both ends using an Illumina Genome Analyzer IIx platform according to the standard Illumina protocols.

Multiplexed libraries were sequenced for 72 bp at both ends using an Illumina Genome Analyzer IIx platform according to the standard Illumina protocols [ 29].

The inserts of randomly chosen clones from these 3 cDNA libraries were sequenced from both ends using an ABI 3730 DNA Analyzer (Applied Biosystems).

Telomere sequencing was done by the chain termination method (19) with primer #4 (5'GTATAATAAAATGAcomplementaryomplementory to nucleotide sequence between the positions 171 to 192 inward from ITR both ends, using an automated sequencer (ABI373S, PE, Applied Biosystems) and a Dye Terminator Cycle Sequencing Ready Reaction kit (PE Applied Biosystems).

If both ends use a high-speed connection like a cable modem or D.S.L., there's only a hint of sluggishness; you may feel as though you're back on the old PC you gave your in-laws in 1998.