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So the GC skew is in opposite directions at both ends of genes.
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Indeed, there are few differences between A. thaliana and rice in internal regions of genes for both codon and amino acid frequencies whereas a strong difference for both is evidenced at both ends of the genes (fig. 8 C and D).
We also globally mapped the 5′- and 3′-boundaries of H. sinensis genes by searching for a sharp reduction of RNA-Seq reads signals at both ends of annotated genes.
It works by adding a particular DNA sequence to both ends of a gene, so that all genes have the same flanks.
Transcription initiation usually occurs from the NDRs at both ends of the genes.
Due to nucleotide nibbling at both ends of D gene segments during recombination, TRBD gene identification is challenging for a mature CDR3 sequence.
At the FBA1 gene, changes were observed in the npl3-S411A and rna15-2 mutatts at both ends of the gene denoting a terminating effect in the flanking MPE1 gene (Figure 5A).
Few reasons for such ambiguity include the impact of partial sequences in databases due to the difficulty in covering both ends of the gene with universal primers commonly used for determining 16S rRNA gene sequences.
The novel and known variants appear to gather at both ends of MTDH gene, with 3 novel variants displayed in the central part of the gene in case group, but not in the control group.
Both ends of this gene share homology with the EcoPI-ModD2 or EcoPI-ModD3 (in N. lactamica) modification gene, while no homology was found at the middle of the gene among the three ModD types (fig. 7).
In contrast, although the expanded GAA repeat tract plus ∼6 kb of flanking sequences in the first intron of the FXN gene is associated with methylated H3K9 in FRDA cells, spreading of this heterochromatic mark towards both ends of the gene seems to be prevented by a mechanism that has yet to be determined.
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